ABSTRACT
Objective: To study the secondary metabolites of endophytic fungus Chaetomium sp. Methods: The compounds were isolated and purified by chromatographic methods on silica gel, Sephadex-LH20 columns, and semi-prep HPLC. The structures were established on the basis of spectroscopic data interpretation. Results: Ten compounds were isolated and identified as chetoseminudin B (1), gonytolide C (2), chaetone D (3), 1H-indole-3-carbaldehyde (4), 1H-indole-3-carboxylic acid (5), indole-3-methylethanoate (6), 3R*,4R*-dihydroxy-3-methylpentan-2-one (7), emodin (8), 3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (9), and (3β,5α, 6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol (10). Conclusion: All compounds are isolated from Chaetomium sp. for the first time.
ABSTRACT
AIM@#To study the chemical constituents from the fermentation of the endophytic fungus HP-1 of Chinese eaglewood.@*METHODS@#The chemical constituents were isolated by column chromatography on silica gel and Sephadex LH-20, and their structures were elucidated on the basis of spectroscopic analysis.@*RESULTS@#Four compounds were isolated and identified as 3α, 3β, 10β-trimethyl-decahydroazuleno[6, 7]furan-8, 9, 14-triol (1), 4-hydroxyphenylacetic acid (2), 4-hydroxyphenethyl alcohol (3), and 5-hydroxymethyl-2-furancarboxaldehyde (4).@*CONCLUSION@#Compound 1 was a new compound. Compound 2 showed antibacterial activity against Staphylococcus aureus.
Subject(s)
Anti-Bacterial Agents , Chemistry , Pharmacology , Endophytes , Chemistry , Fungi , Chemistry , Phenylacetates , Chemistry , Pharmacology , Sesquiterpenes , Chemistry , Thymelaeaceae , Microbiology , Wood , MicrobiologyABSTRACT
A fungus CQ31 isolated from soil samples was identified as Chaetomium sp.. This strain produced effectively xylanases utilizing several liguocellulosic materials in the solid-state fermentation (SSF), and corn straw was the best carbon source. The results of single-factor-experiment showed that the corn straw as the carbon source, tryptone as the nitrogen source, initial moisture content of 80% and initial pH 9.0 were the optimal conditions for xylanase production. Under the optimized conditions, it produced xylanase which was 4897 U/g dry substrate while mannanase was 803 U/g dry substrate after 7 days of cultivation. Therefore, xylanase and mannanase production by Chaetomium sp. CQ31 in SSF possess potential for commercial applications.